Recombinant Human Interleukin-3 GMP (rHuIL-3)
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
Less than 0.01 EU/ug of rHuIL-3 GMP as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-41.
> 98 % by SDS-PAGE and HPLC analyses.
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