英文全名

Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A)

英文别名

TNFRSF12A, FGF-inducible 14, CD266

蛋白结构

Squence of Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A

氨基酸序列

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Accession

Q9NP84

Gene ID

51330

产品英文概述

来源

Escherichia coli.

分子量

Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuTWEAK Receptor as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86.2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51.3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33.4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A

包装规格

5ug/100ug/500ug

安全数据表下载 (SDS)

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品牌链接

Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human TNF-related Weak Inducer of Apoptosis Receptor/TNFRSF12A (rHuTWEAKReceptor/TNFRSF12A)