英文全名

Recombinant Murine Interferon-gamma (rMuIFN-gamma)

英文别名

蛋白结构

Squence of Recombinant Murine Interferon-gamma

氨基酸序列

HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC

Accession

P01580

Gene ID

15978

产品英文概述

来源

Escherichia coli.

分子量

Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, containing 5 % trehalose.

内毒素

Less than 1 EU/ug of rMuIFN-gamma as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, 2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.

纯度

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Murine Interferon-gamma

包装规格

100ug/500ug

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品牌链接

Recombinant Murine Interferon-gamma是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Murine Interferon-gamma (rMuIFN-gamma)