英文全名

Recombinant Murine Interleukin-10 (rMuIL-10)

英文别名

CSIF

蛋白结构

Squence of Recombinant Murine Interleukin-10

氨基酸序列

SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS

Accession

P18893

Gene ID

16153

产品英文概述

Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2). The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Ralpha/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature murine IL-10 shares 70 %-77 % amino acid sequence identity with human, bovine, porcine IL-10, but murine IL-10 does not act on human cells.

来源

Escherichia coli.

分子量

Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rMuIL-10 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Murine Interleukin-10

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Murine Interleukin-10是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Murine Interleukin-10 (rMuIL-10)