英文全名

Recombinant Murine Keratinocyte Growth Factor-2/FGF-10 (rMuKGF-2/FGF-10)

英文别名

蛋白结构

Squence of Recombinant Murine Keratinocyte Growth Factor-2/FGF-10

氨基酸序列

QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT

Accession

O35565

Gene ID

14165

产品英文概述

来源

Escherichia coli.

分子量

Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol.

内毒素

Less than 1 EU/ug of rMuKGF-2/FGF-10 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Murine Keratinocyte Growth Factor-2/FGF-10

包装规格

5ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Murine Keratinocyte Growth Factor-2/FGF-10是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Murine Keratinocyte Growth Factor-2/FGF-10 (rMuKGF-2/FGF-10)