英文全名

Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF)

英文别名

ATF

蛋白结构

Squence of Recombinant Rat Glial Cell-derived Neurotrophic Factor

氨基酸序列

SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI

Accession

Q07731

Gene ID

25453

产品英文概述

来源

Escherichia coli.

分子量

Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4.

内毒素

Less than 0.1 EU/ug of rRtGDNF as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2.2. Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80.3. Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7.4. Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Rat Glial Cell-derived Neurotrophic Factor

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Rat Glial Cell-derived Neurotrophic Factor是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF)