Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF)
SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI
Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
Sterile filtered white lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4.
Less than 0.1 EU/ug of rRtGDNF as determined by LAL method.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
1. Lin LF, Doherty DH, Lile JD, et al. 1993. Science. 260:1130-2.2. Russell FD, Koishi K, Jiang Y, et al. 2000. Neuroscience. 97:575-80.3. Jing S, Yu Y, Fang M, et al. 1997. J Biol Chem. 272:33111-7.4. Cik M, Masure S, Lesage AS, et al. 2000. J Biol Chem. 275:27505-12.
> 98 % by SDS-PAGE and HPLC analyses.
Recombinant Rat Glial Cell-derived Neurotrophic Factor是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Rat Glial Cell-derived Neurotrophic Factor (rRtGDNF)