英文全名

Recombinant Rat VEGF Co-regulated Chemokine 1/CXCL17 (rRtVCC-1/CXCL17)

英文别名

Dendritic Cell and Monocyte Chemokine-like Protein, DMC

蛋白结构

Squence of Recombinant Rat VEGF Co-regulated Chemokine 1/CXCL17

氨基酸序列

SPNQEVARHH GDQHQAPRRW LWEGGQECDC KDWSLRVSKR KTTAVLEPPR KQCPCDHVKG SEKKNRRQKH HRKSQRPSRT CQQFLKRCQL ASFTLPL

Accession

Gene ID

产品英文概述

CXCL17 also named Dendritic Cell and Monocyte Chemokine-like Protein (DMC) and VEGF Co-regulated Chemokine 1 (VCC-1), is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed in the lung and can be detected in trachea, stomach and skeletal muscle. CXCL17 attracts dendritic cells and monocytes and is regulated in tumors. It may be a housekeeping chemokine regulating recruitment of nonactivated blood monocytes and immature dendritic cells into tissues and may play a role in the innate defense against infections. Mature human CXCL17 shares 73 %, 71 % and 64 % amino acid sequence identity with bovine, mouse and rat DMC, respectively.

来源

Escherichia coli.

分子量

Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 ug/ml, corresponding to a specific activity of > 200 IU/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rRtVCC-1/CXCL17 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. WY Lee, CJ Wang, TY Lin, et al. 2013. Am J Physiol Endocrinol Metab, 304: E32-40.2. A Matsui, H Yokoo, Y Negishi, et al. 2012. PLoS One, 7: e44080.3. AM Burkhardt, KP Tai, JP Flores-Guiterrez, et al. 2012. J Immunol, 188: 6399-406.4. N Hiraoka, R Yamazaki-Itoh, Y Ino, et al. 2011. Gastroenterology, 140: 310-21.

纯度

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Rat VEGF Co-regulated Chemokine 1/CXCL17

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

点击下载

品牌链接

Recombinant Rat VEGF Co-regulated Chemokine 1/CXCL17是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Rat VEGF Co-regulated Chemokine 1/CXCL17 (rRtVCC-1/CXCL17)