英文全名

Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10)

英文别名

蛋白结构

Squence of Recombinant Human Keratinocyte Growth Factor-2/FGF-10

氨基酸序列

LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

Accession

O15520

Gene ID

2255

产品英文概述

来源

分子量

Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuKGF-2/FGF-10 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7.

纯度

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Keratinocyte Growth Factor-2/FGF-10

包装规格

5ug/100ug/500ug

安全数据表下载 (SDS)

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品牌链接

Recombinant Human Keratinocyte Growth Factor-2/FGF-10是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10)

英文全名

Recombinant PreScission Protease (rPPase)

英文别名

3C protease, Picornain 3C, PSP

蛋白结构

Squence of Recombinant PreScission Protease

氨基酸序列

Accession

Gene ID

产品英文概述

PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease. rPP is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein. rPP works most effective at 4°C and can digest substrates at room temperature as well.

来源

Escherichia coli.

分子量

PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.

生物活性

外观

Sterile colorless liquid.

配方

50 mM Tris-HCl, pH 7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use.

内毒素

溶解说明

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

参考资料

1. Werner G, Rosenwirth B, Bauer E, et al. 1986. J Virol, 57: 1084-93.2. Libby RT, Cosman D, Cooney MK, et al. 1988. Biochemistry, 27: 6262-8.3. Aschauer B, Werner G, McCray J, et al. 1991. Virology, 184: 587-94.4. Leong LE, Walker PA, Porter AG. 1993. J Biol Chem, 268: 25735-9.

纯度

One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90 % completion at 5 °C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

SDS-PAGE电泳

SDS-PAGE of Recombinant PreScission Protease

包装规格

100IU/250IU

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品牌链接

Recombinant PreScission Protease是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant PreScission Protease (rPPase)

英文全名

Recombinant Human Apolipoprotein-Serum Amyloid A1 (rHuApo-SAA1)

英文别名

蛋白结构

Squence of Recombinant Human Apolipoprotein-Serum Amyloid A1

氨基酸序列

RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY

Accession

P0DJI8

Gene ID

6288

产品英文概述

来源

分子量

Approximately 11.7 kDa, a single non-glycosylated polypeptide chain containing 104 amino acids.

生物活性

Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 9.0, 150 mM NaCl.

内毒素

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Apolipoprotein-Serum Amyloid A1

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Apolipoprotein-Serum Amyloid A1是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Apolipoprotein-Serum Amyloid A1 (rHuApo-SAA1)

英文全名

Recombinant Streptavidin (rStreptavidin)

英文别名

蛋白结构

Squence of Recombinant Streptavidin

氨基酸序列

MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS

Accession

P22629

Gene ID

产品英文概述

来源

分子量

~52,000 per tetramer

生物活性

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized in 10 mM potassium phosphate buffer pH 6.5.

内毒素

Less than 0.1 EU/ug of rStreptavidin as determined by LAL method.

溶解说明

Dissolve with double distilled water

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- Refer to lot specific COA for the Use by Date when stored at ≤ -20 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses

SDS-PAGE电泳

SDS-PAGE of Recombinant Streptavidin

包装规格

1mg/25mg/100mg

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Streptavidin是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Streptavidin (rStreptavidin)

英文全名

Recombinant Human Interferon-gamma (rHuIFN-gamma)

英文别名

蛋白结构

Squence of Recombinant Human Interferon-gamma

氨基酸序列

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ

Accession

P01579

Gene ID

3458

产品英文概述

Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-beta or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments TH1 cell expansion and may be required for TH1 cell differentiation.

来源

分子量

Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuIFN-gamma as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, 2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Interferon-gamma

包装规格

100ug/500ug

安全数据表下载 (SDS)

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品牌链接

Recombinant Human Interferon-gamma是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Interferon-gamma (rHuIFN-gamma)

英文全名

Recombinant Human Tumor Necrosis Factor-beta/TNFSF1 (rHuTNF-beta/TNFSF1)

英文别名

蛋白结构

Squence of Recombinant Human Tumor Necrosis Factor-beta/TNFSF1

氨基酸序列

Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of > 2.0 × 108 IU/mg in the presence of actinomycin D.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, with 0.02 % Tween-20.

内毒素

Less than 0.1 EU/ug of rHuTNF-beta/TNFSF1 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Tumor Necrosis Factor-beta/TNFSF1

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Tumor Necrosis Factor-beta/TNFSF1是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Tumor Necrosis Factor-beta/TNFSF1 (rHuTNF-beta/TNFSF1)

英文全名

Recombinant Human Epidermal Growth Factor, 1-51a.a. (rHuEGF,1-51a.a.)

英文别名

蛋白结构

Squence of Recombinant Human Epidermal Growth Factor, 1-51a.a.

氨基酸序列

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E

Accession

P01133

Gene ID

1950

产品英文概述

来源

分子量

Approximately 6.0 kDa, a single glycosylated polypeptide chain containing 51 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.1 EU/ug of rHuEGF, 1-51a.a. as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Epidermal Growth Factor, 1-51a.a.

包装规格

100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Epidermal Growth Factor, 1-51a.a.是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Epidermal Growth Factor, 1-51a.a. (rHuEGF,1-51a.a.)

英文全名

Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade)

英文别名

LR3 IGF-I

蛋白结构

Squence of Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade

氨基酸序列

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Accession

Gene ID

产品英文概述

IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.

来源

Escherichia coli.

分子量

Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.

生物活性

Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 U/mg.
Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 U/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.2.

内毒素

Less than 0.01 EU/ug of rHuLR3 IGF-1 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.-Refer to lot specific COA for the Use by Date when stored at 2 to 8 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Conlon MA, Tomas FM, Owens PC, et al. 1995. J Endocrinol, 146: 247-53.2. Dunaiski V, Dunshea FR, Walton PE, et al. 1997. J Endocrinol, 155: 559-65.3. Garnaut SM, Howarth GS, Read LC. 2002. Growth Factors, 20: 17-25.4. Xi G, Kamanga-Sollo E, Pampusch MS, et al. 2004. J Cell Physiol, 200: 387-94.5. Hadsell DL, Parlow AF, Torres D, et al. 2008. J Endocrinol, 198: 61-70.

纯度

> 98 % by SDS-PAGE analyses.>90 % by RP-HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade

包装规格

1mg/5mg/20mg

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade)

英文全名

Recombinant Human Fibroblast Growth Factor 21 (rHuFGF-21)

英文别名

蛋白结构

Squence of Recombinant Human Fibroblast Growth Factor 21

氨基酸序列

HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S

Accession

Q9NSA1

Gene ID

26291

产品英文概述

来源

分子量

Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ug/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 ug/ml of rMuKlotho-beta and 10 ug/ml of heparin.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuFGF-21 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Fibroblast Growth Factor 21

包装规格

5ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Fibroblast Growth Factor 21是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Fibroblast Growth Factor 21 (rHuFGF-21)

英文全名

Recombinant Human Fibroblast Growth Factor 19 (rHuFGF-19)

英文别名

蛋白结构

Squence of Recombinant Human Fibroblast Growth Factor 19

氨基酸序列

MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK

Accession

O95750

Gene ID

9965

产品英文概述

来源

分子量

Approximately 21.8 kDa, a single non-glycosylated polypeptide chain containing 195 amino acids.

生物活性

Assay #1: Fully biologically active when compared to standard. The biological activity is measured by its binding ability in a functional ELISA. Immobilized rHuFGF R4 at 5 ug/ml can bind rHuFGF-19 with a linear range of 3-200 ng/ml.Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuFGF-19 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Ladher RK, Anakwe KU, Gurney AL, et al. 2000. Science. 290:1965-7.2. Tomlinson E, Fu L, John L, et al. 2002. Endocrinology. 143:1741-7.3. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7.4. Fu L, John LM, Adams SH, et al. 2004. Endocrinology. 145:2594-603.5. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.6. Kurosu H, Kuro OM. 2009. Mol Cell Endocrinol. 299:72-8.7. Lin BC, Wang M, Blackmore C, et al. 2007. J Biol Chem. 282:27277-84.8. Kharitonenkov A, Dunbar JD, Bina HA, et al. 2008. J Cell Physiol. 215:1-7.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Fibroblast Growth Factor 19

包装规格

5ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Fibroblast Growth Factor 19是ChemWhat品牌授权产品并通过沃尔森销售,下面是ChemWhat上的该产品链接 Recombinant Human Fibroblast Growth Factor 19 (rHuFGF-19)