ChemWhat重组蛋白归档 - 第57页共57页 - Watson(沃森)

ChemWhat重组蛋白, 产品

Recombinant Human Insulin-like Growth factor-1 GMP (rHuIGF-1)

英文全名

Recombinant Human Insulin-like Growth factor-1 GMP (rHuIGF-1)

英文别名

Somatomedin C, IGF-I, IGF-IA

蛋白结构

氨基酸序列

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Accession

Gene ID

产品英文概述

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94 % and 96 % a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.

来源

Escherichia coli.

分子量

Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.

内毒素

Less than 0.01 EU/ug of rHuIGF-1 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Skottner A, Fryklund L, Hansson HA. 1986. Acta Paediatr Scand Suppl, 325: 107-11.2. Bartlett WP, Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.3. Palmade F, Sechoy-Chambon O, Coquelet C, et al. 1994. Curr Eye Res, 13: 531-7.4. Tennagels N, Hube-Magg C, Wirth A, et al. 1999. Biochem Biophys Res Commun, 260: 724-8.5. Laron Z. 2004. Novartis Found Symp, 262: 56-77; discussion -83, 265-8.6. Shiratsuchi I, Akagi Y, Kawahara A, et al. 2011. Anticancer Res, 31: 2541-5.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Insulin-like Growth factor-1 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Insulin-like Growth factor-1 GMP (rHuIGF-1)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Basic Fibroblast Growth Factor GMP (rHubFGF)

英文全名

Recombinant Human Basic Fibroblast Growth Factor GMP (rHubFGF)

英文别名

FGF-2, HBGF-2

蛋白结构

氨基酸序列

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Accession

Gene ID

产品英文概述

Human bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named “acidic fibroblast growth factor” (FGF-1) and “basic fibroblast growth factor” (FGF-2). Human bFGF shares 54 % amino acid sequence identity with aFGF. Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

来源

Escherichia coli.

分子量

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.

内毒素

Less than 0.01 EU/ug of rHubFGF GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.2. Gospodarowicz D. 1974. Nature. 249:123-7.3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Basic Fibroblast Growth Factor GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Basic Fibroblast Growth Factor GMP (rHubFGF)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP (rHuFlt-3Ligand)

英文全名

Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP (rHuFlt-3Ligand)

英文别名

Flt3L, SL Cytokine

蛋白结构

氨基酸序列

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

Accession

Gene ID

产品英文概述

Flt3-ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt3. Flt3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt3-ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72 % identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

来源

Escherichia coli.

分子量

Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 155 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.

内毒素

Less than 0.01 EU/ug of rHuFlt3-Ligand GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Hacein-Bey S, Basile GD, Lemerle J, et al. 1998. Blood, 92: 4090-7.2. Peters M, Solem F, Goldschmidt J, et al. 2001. Exp Hematol, 29: 146-55.3. Beq S, Fontanet A, Theze J, et al. 2004. AIDS, 18: 2089-91.4. Mahadevan D, Choi J, Cooke L, et al. 2009. Hum Genomics Proteomics, 2009: 453634.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP (rHuFlt-3Ligand)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 GMP (rHuTNF-alpha/TNFSF2)

英文全名

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 GMP (rHuTNF-alpha/TNFSF2)

英文别名

Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin

蛋白结构

氨基酸序列

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Accession

Gene ID

产品英文概述

Tumor necrosis factor alpha (TNF-alpha), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-alpha occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Human and murine TNF-alpha show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-alpha have been described and virtually all cell types studied show the presence of one or both of these receptor types.

来源

Escherichia coli.

分子量

Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 10 mM PB, 10 mM NaCl, pH 7.0.

内毒素

Less than 0.01 EU/ug of rHuTNF-α/TNFSF2 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2 GMP (rHuTNF-alpha/TNFSF2)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor GMP (rHuGM-CSF)

英文全名

Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor GMP (rHuGM-CSF)

英文别名

Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α

蛋白结构

氨基酸序列

APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Accession

Gene ID

产品英文概述

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. Human GM-CSF shares 54 % sequences identity with mouse GM-CSF, but has no biological effects across species. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy and has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients.

来源

Escherichia coli.

分子量

Approximately 14.5 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.01 EU/ug of rHuGM-CSF GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.2. 1989. N Engl J Med, 320: 253-4.3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor GMP (rHuGM-CSF)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Interleukin-15 GMP (rHuIL-15)

英文全名

Recombinant Human Interleukin-15 GMP (rHuIL-15)

英文别名

IL-T

蛋白结构

氨基酸序列

NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS

Accession

Gene ID

产品英文概述

Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97 % and 73 % sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone.

来源

Escherichia coli.

分子量

Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.01 EU/ug of rHuIL-15 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Anderson DM, Johnson L, Glaccum MB, et al. 1995. Genomics, 25: 701-6.2. Krause H, Jandrig B, Wernicke C, et al. 1996. Cytokine, 8: 667-74.3. Chirifu M, Hayashi C, Nakamura T, et al. 2007. Nat Immunol, 8: 1001-7.4. Grabstein KH, Eisenman J, Shanebeck K, et al. 1994. Science, 264: 965-8.5. Giri JG, Ahdieh M, Eisenman J, et al. 1994. EMBO J, 13: 2822-30.6. Arena A, Merendino RA, Bonina L, et al. 2000. New Microbiol, 23: 105-12.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Interleukin-15 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interleukin-15 GMP (rHuIL-15)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Interleukin-7 GMP (rHuIL-7)

英文全名

Recombinant Human Interleukin-7 GMP (rHuIL-7)

英文别名

LP-1, pre-B cell factor

蛋白结构

氨基酸序列

DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH

Accession

Gene ID

产品英文概述

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-speciesactivity. IL-7 as an immunotherapy agent has been examinedin many human clinical trials for various malignancies and during HIV infection.

来源

Escherichia coli.

分子量

Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

生物活性

Assay #1:Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Assay #2: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL) is 0.1-0.5 ng/mL. The specific activity of Recombinant Human IL-7 is approximately 2.0 × 108 units/mg, which is calibrated against human IL-7 WHO Standard (NIBSC code: 90/530).

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.01 EU/ug of rHuIL-7 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6.2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2.3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80.5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904.6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Interleukin-7 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interleukin-7 GMP (rHuIL-7)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Interleukin-4 GMP (rHuIL-4)

英文全名

Recombinant Human Interleukin-4 GMP (rHuIL-4)

英文别名

BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra.

蛋白结构

氨基酸序列

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Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

Approximately 15.0 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.01 EU/ug of rHuIL-4 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Sokol CL, Barton GM, Farr AG, et al. 2008. Nat Immunol. 9:310-8.2. Hershey GK, Friedrich MF, Esswein LA, et al. 1997. N Engl J Med. 337:1720-5.3. Yokota T, Otsuka T, Mosmann T, et al. 1986. Proc Natl Acad Sci U S A. 83:5894-8.4. Eder A, Krafft-Czepa H, Krammer PH. 1988. Nucleic Acids Res. 16:772.5. Walter MR, Cook WJ, Zhao BG, et al. 1992. J Biol Chem. 267:20371-6.

纯度

> 98 % by SDS-PAGE analyses. > 95 % by HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Interleukin-4 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interleukin-4 GMP (rHuIL-4)

2021年12月23日/通过： great_watson-int