英文全名

Recombinant Human Secreted Protein Acidic and Rich in Cysteine, His (rHuSPARC,His)

英文别名

Basement-membrane Protein 40, BM-40, Osteonectin, ON

蛋白结构

氨基酸序列

MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI

Accession

P09486

Gene ID

6678

产品英文概述

来源

Escherichia coli.

分子量

Approximately 36.1 kDa, a single non-glycosylated polypeptide chain containing 314 amino acids, with expression vector sequence (containing 6 × His tag).

生物活性

Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuSPARC, His as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Sage H, Decker J, Funk S, et al. 1989. J Mol Cell Cardiol, 21 Suppl 1: 13-22.2. Kanauchi M, Nishioka M, Dohi K. 2000. Diabetologia, 43: 1076-7.3. Lau CP, Poon RT, Cheung ST, et al. 2006. J Pathol, 210: 459-68.4. Rodriguez-Jimenez FJ, Caldes T, Iniesta P, et al. 2007. Oncol Rep, 17: 1301-7.5. Wong SY, Crowley D, Bronson RT, et al. 2008. Clin Exp Metastasis, 25: 109-18

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Secreted Protein Acidic and Rich in Cysteine, His是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Secreted Protein Acidic and Rich in Cysteine, His (rHuSPARC,His)

英文全名

Recombinant Human Ubiquitin-conjugating Enzyme E2 D3, His (rHuUBE2D3,His)

英文别名

Ubiquitin Carrier Protein D3, Ubiquitin-protein Ligase D3

蛋白结构

氨基酸序列

MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM

Accession

P61077

Gene ID

7323

产品英文概述

Ubiquitin-conjugating enzyme E2 D3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2D3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The ubiquitination reaction can modify proteins and regulate protein degradation. The UBE2D3 is a human homolog of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. It mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, IκBalpha, p105) and abnormal proteins and has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.

来源

Escherichia coli.

分子量

Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids (a.a.) of human UBE2D3/UBC5C and 8 a.a. vector sequence including 6 × His tag at N-terminus.

生物活性

No data available

外观

Sterile Colorless liquid.

配方

A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 6.5, with 125 mM NaCl, 10 % Glycerol, 5 % Trehalose, 1 mM DTT.

内毒素

Less than 1 EU/ug of rHuUBE2D3/UBC5C, His as determined by LAL method.

溶解说明

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

点击下载

品牌链接

Recombinant Human Ubiquitin-conjugating Enzyme E2 D3, His是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Ubiquitin-conjugating Enzyme E2 D3, His (rHuUBE2D3,His)

英文全名

Recombinant Flagellin, His (rFlagellin,His)

英文别名

蛋白结构

氨基酸序列

MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH

Accession

P06179

Gene ID

1253480

产品英文概述

来源

Escherichia coli.

分子量

Approximately 52.7 kDa, a single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus.

生物活性

No data available

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rFlagellin, His as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Fedorov OVandKostyukova AS. 1984. FEBS Lett, 171: 145-8.2. Belas RandFlaherty D. 1994. Gene, 148: 33-41.3. Givaudan A, Lanois A, Boemare N. 1996. Gene, 183: 243-53.4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12: 1783-94.5. Hayashi F, Smith KD, Ozinsky A, et al. 2001. Nature, 410: 1099-103.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

点击下载

品牌链接

Recombinant Flagellin, His是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Flagellin, His (rFlagellin,His)

英文全名

Recombinant Hirudin (rHirudin)

英文别名

蛋白结构

氨基酸序列

VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL

Accession

P84590

Gene ID

产品英文概述

来源

Pichia Pastoris

分子量

Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.

生物活性

The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.

内毒素

Less than 1 EU/ug of rHirudin as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Knapp A, Degenhardt T, Dodt J. 1992. J Biol Chem, 267: 24230-4.2. Pineo GFandHull RD. 1995. Curr Opin Hematol, 2: 380-5.3. Badimon L, Merino A, Badimon J, et al. 1991. Trends Cardiovasc Med, 1: 261-7.4. Nasir MA, Toth CA, Mittra RA. 1996. Am J Ophthalmol, 121: 554-60.5. Agnelli GandSonaglia F. 1997. Semin Thromb Hemost, 23: 143-8.

纯度

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

点击下载

品牌链接

Recombinant Hirudin是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Hirudin (rHirudin)

英文全名

Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP (rHuFlt-3Ligand)

英文别名

Flt3L, SL Cytokine

蛋白结构

氨基酸序列

TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA

Accession

P49771

Gene ID

2323

产品英文概述

Flt3-ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt3. Flt3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt3-ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. An alternatively-spliced FL mRNA, encoding a soluble form of the human FL, has also been identified. FL is widely expressed in various human and mouse tissues. At the amino acid sequence level, human and mouse FL are approximately 72 % identical and the two proteins exhibit cross-species activity. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

来源

Escherichia coli.

分子量

Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 155 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.

内毒素

Less than 0.01 EU/ug of rHuFlt3-Ligand GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Hacein-Bey S, Basile GD, Lemerle J, et al. 1998. Blood, 92: 4090-7.2. Peters M, Solem F, Goldschmidt J, et al. 2001. Exp Hematol, 29: 146-55.3. Beq S, Fontanet A, Theze J, et al. 2004. AIDS, 18: 2089-91.4. Mahadevan D, Choi J, Cooke L, et al. 2009. Hum Genomics Proteomics, 2009: 453634.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

点击下载

品牌链接

Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Fms-related Tyrosine Kinase 3 Ligand GMP (rHuFlt-3Ligand)